From Organelle to Protein Gel: A 6-Wk Laboratory Project on Flagellar Proteins

A New Approach to Detect Similar Proteins from 2D Gel

A SAS-6-Like Protein Suggests that the Toxoplasma Conoid Complex Evolved from Flagellar Components

SAS-6 is required for centriole biogenesis in diverse eukaryotes. Here, we describe a novel family of SAS-6-like (SAS6L) proteins that share an N-terminal domain with SAS-6 but lack coiled-coil tails. SAS6L proteins are found in a subset of eukaryotes that contain SAS-6, including diverse protozoa and green algae. In the apicomplexan parasite Toxoplasma gondii, SAS-6 localizes to the centriole ...

The effects of 12-wk combined aerobic/resistance training on C-reactive protein (CRP) serum and interleukin-6 (IL-6) plasma in sedentary men

Background : The purpose of this study was to examine the influence of combined training program on C-reactive protein (CRP) serum and interleukin-6 (IL-6) plasma concentrations in sedentary men. Materials and Methods: In this quasi-experimental study, twenty sedentary men (23±2 years) volunteers (with no history of regular exercise program, weight deviation more than 2 kg, special disease and...

Antigenic analysis of Campylobacter flagellar protein and other proteins.

Outer membrane proteins of Campylobacter jejuni and other campylobacter species were analyzed for their antigenic potentials by immunoblotting. Polypeptides were resolved by sodium dodecyl sulfate-polyacrylamide gel electrophoresis, transferred electrophoretically, and reacted with rabbit antisera to C. jejuni. Each Campylobacter species analyzed demonstrated a unique outer membrane protein ant...

The bacterial flagellar protein export apparatus processively transports flagellar proteins even with extremely infrequent ATP hydrolysis

For self-assembly of the bacterial flagellum, a specific protein export apparatus utilizes ATP and proton motive force (PMF) as the energy source to transport component proteins to the distal growing end. The export apparatus consists of a transmembrane PMF-driven export gate and a cytoplasmic ATPase complex composed of FliH, FliI and FliJ. The FliI(6)FliJ complex is structurally similar to the...